Composite

Part:BBa_K4642034:Design

Designed by: Ryan Yapa   Group: iGEM23_City-of-London-UK   (2023-09-28)


T7 / Gen4 320b-320a-4640 Double AND-Gate / mCerulean / Double Terminator / T7 / Gen4 320b-320a-4640


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 216
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A riboregulator that activates gene expression must switch from a secondary structure that prevents translation to a configuration that promotes translation upon binding of a cognate transacting RNA. Although the Shine-Dalgarno sequence is an important factor in determining the efficiency of translation from a given RNA, studies have found that secondary structure in regions nearby the start codon also plays a critical role. Furthermore, genome-wide analyses have revealed strong biases toward low secondary structures around the start codon of mRNAs from a panel of hundreds of bacterial genomes. Thus, we ensured that toehold switches sequestered the region around the start codon to repress translation, rather than binding to either the RBS or the start codon.


Source

Custom designed in-silico using NUPACK API.

References